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Purpose: This study aimed to explore the factors influencing attitudes toward withdrawal of life-sustaining treatment (WLST) in adolescents. Methods: This study used a descriptive research design. A structured questionnaire was administered to 185 adolescents. Data were analyzed based on t-tests, analysis of variance, Pearson’s correlation coefficient, and multiple regression using the IBM SPSS 20.0. Statistical package. Results: There was a negative correlation between attitudes toward WLST and respect for life (r=-.188, p=.010). Knowing about life-sustaining treatment (β=-0.30, p<.001), experience of WLST education (β=0.21, p=.003), experience of respect for life education (β=-0.16, p=.021), and experience of death (family or acquaintance) (β=-0.13, p=.039) explained 16.7% (F=8.39, p<.001) of the variance in attitudes toward WLST. The respect for life did not affect attitudes toward WLST (β=-0.07, p=.347). Conclusion: The study findings suggest that WLST in adolescents is different from that of adults. Further research is needed to develop strategies for sound and positive attitudes toward WLST in adolescents.