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Hydrogen pre-charged Zr-1.0Nb alloy was prepared to study the effect of hydrogen uptake on its mechanical properties. The apparent activation energy of the Zr-1.0Nb alloy for hydriding was about 27 kcal/mol. The tensile strength of the Zr-1.0Nb alloy with a hydrogen content of up to 700 ppm was similar to that of an as-fabricated specimen, in that its elongation was drastically decreased after 400 ppm and became nearly zero above about 1200 ppm. The critical hydrogen content range needed to produce the ductile-brittle transition was above 400 ppm.