초록 close

Background: This study examined the osteoinductive activity of demineralized dentin matrix (DDM) from humanand polydeoxyribonucleotide (PDRN) for nude mice. Methods: Twenty healthy nude mice, weighing about 15~20 g, were used for the study. DDM from human andPDRN were prepared and implanted subcutaneously into the dorsal portion of the nude mice. The nude mice weresacrificed at 1, 2, and 4 weeks after grafting and evaluated histologically by hematoxylin-eosin and Masson’strichrome staining. The specimens were also evaluated via a histomorphometric study. Results: The DDM and PDRN induced new bone, osteoblasts, and fibroblasts in soft tissues. The histological findingsshowed bone-forming cells like osteoblasts and fibroblasts at 1, 2, and 4 weeks. New bone formation was observed inthe histomorphometric study. In particular, the ratio of new bone formation was the highest at 2 weeks comparedwith the first week and fourth week. Conclusions: In this study, we showed that the PDRN used in this experimental model was able to induce boneregeneration when combined to the DDM.