초록 close

Epithelial cells are known to be unstable at different passages, which is one of the big obstacles in hair regeneration. In this study, the effect of a newly developed scaffold composed of alginate and BSM (bladder submucosa matrix) on the adhesion and proliferation of hair epithelial cells was investigated. BSM is an extracellular matrix and known to have various types of growth factors, adhesion molecules, fibrin, albumin, lamina-A/C, keratin 1 and keratin 2A which enhance cell adhesion and proliferation. The BSM-alginate composite was fabricated into a sponge type scaffold by a freeze drying method. Porosity, structural morphology water absorption capacity, cell adhesion property and cytotoxicity of the scaffold were characterized. The results demonstrated that BSM-alginate sponge provided proper physical environment for proliferation of epithelial cells at early stage, indicating the potential of this composite scaffold as a biomaterial for hair regeneration.