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The purpose of this study was to explore the relationship between the gender role identity and intrinsic motivation of female university students. 57 female university exercisers and 113 non-exercisers were recruited and two questionnaires (Bem Sex Role Inventory, BSRI; Intrinsic Motivation Inventory, IMI) were administered. Statistical analyses revealed that 1) masculinity was higher than femininity in exercise participation frequency; 2) masculinity influenced positively in all factors except tension-anxiety factor of intrinsic motivation; and 3) masculinity was higher in intrinsic motivation than femininity/androgyny in the difference between gender role identity and intrinsic motivation. These results were discussed based upon theoretical back ground of gender role identity and behavioral prospects in association with intrinsic motivation.