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In this study, sequence-related amplified polymorphism (SRAP), amplified fragment length polymorphism (AFLP), and randomly amplified polymorphic DNA (RAPD) analyses were used to assess genetic diversity among 64 peach germplasms including commercial cultivars and wild accessions. Nineteen SRAP primer combinations were detected a total of 143 polymorphic bands (26.7%) with an average 7.53. In AFLP analysis, twenty primer combinations were generated a total of 193 polymorphic bands (27.2%) with an average 9.65. Fifty-two RAPD primers were detected a total of 201 polymorphic bands (45.9%) with an average of 3.90. By UPGMA (unweighted pair-group method arithmetic average) cluster analysis with 537 polymorphic bands,the peach germplasms were classified eight groups by similarity index of 0.751. Genetic similarity values among the tested peach germplasms ranged from 0.370 to 0.978, and the average similarity value was 0.737. The similarity index was the highest (0.978)between PH065 and PH066, and the lowest (0.370) between Prunus davidiana and PH020. The genetic diversity among the 64peach germplasms revealed by cluster analysis was low, despite they were collected from different regions.