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오가피(Acanthopanax sessiliflorus Seeman) 열매를 실온에서70% ethanol (EtOH)로 추출하고 이 추출물을 ethyl acetate (EtOAc) 분획, n-butyl alcohol 분획, H2O 분획으로 나누었다. EtOAc 분획에 대하여 silica gel, octadecyl silica gel 및Sephadex LH-20 column chromatography를 반복 실시하여 2종의 화합물을 분리, 정제하였다. NMR, infrared spectroscopy, 및electron ionization/mass spectrometry 등의 spectrum을 해석하여, 화합물 1과 화합물 2를 각각 3,5-dihydroxycinnamic acid과protocatechuic acid 로 구조를 결정하였다. 화합물 1은 오가자에서는 처음으로 분리된 화합물이다. 또한 이 화합물에 대한2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)diammonium salt, 1,1-diphenyl-2-picrylhydrazyl 및 oxygen radical absorbance capacity radical 소거능을 이용한 항산화 활성을 측정하였는데,모두 vitamin C보다 2배 이상 활성이 높은 것으로 나타났다.


The fruits of Acanthopanax sessiliflorus Seeman (Araliaceae) were extracted with 70% aqueous ethanol at room temperature. The concentrated extract was partitioned with ethyl acetate (EtOAc), n-butyl alcohol, and H2O, successively. From the EtOAc fraction, two compounds were isolated through the repeated silica gel, octadecyl silica gel, and Sephadex LH-20column chromatographies. According to the results of physicochemical and spectroscopic data including NMR, mass spectrometry,and infrared spectroscopy, the chemical structures of the compounds were determined as 3,5-dihydroxycinnamic acid (1)and protocatechuic acid (2). Compound 1 was isolated from the fruits of A. sessiliflirus Seeman for the first time. And the compounds were evaluated for the radical scavenging the antioxidant capacity using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)diammonium salt, 1,1-diphenyl-2-picrylhydrazy, and oxygen radical absorbance capacity assay.