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암피실린 (ampicillin, AM) 을 넙치 (평균체중 300g,20±1.0℃) 에 1일 1회 경구 (10, 20, 40㎎/㎏), 근육주사(5, 10, 20㎎/㎏), 약욕 (10, 20, 40ppm; 1시간) 한 다음,경시적 (1시간∼360시간) 으로 혈장 내 AM의 잔류량을 HPLC로써 분석하였다. 10, 20 및 40㎎/㎏로 경구투여한 경우, 최대혈중농도가 모두 10시간째 각각3.62±0.97, 5.20±0.70 및 11.18±0.87㎍/㎖로 나타났으며, 이들은 각각 144, 360 및 360시간째 검출되지 않았다. 5, 10 및 20㎎/㎏의 농도로 근육주사한 경우, 최대혈중농도가 모두 5시간째 6.92±1.29, 9.89±2.22 및19.85±2.97㎍/㎖를 나타내었으며, 이들은 각각 216,264 및 264시간째 혈중에서 검출되지 않았다. 10, 20및 40ppm의 농도로 약욕한 경우, 최대혈중농도가 모두 3시간째 4.39±1.10, 9.57±1.51 및 11.61±1.92㎍/㎖로 나타났으며, 이들은 각각 264, 264 및 360시간째혈중에서 검출되지 않았다. 이로써 경구, 주사 및 약욕 투약에 의한 넙치 혈액 중 분포와 배설정도는 투약량에 의존적 경향이라는 것을 알 수 있었다.


The residue levels of ampicillin (AM) were studied after oral administration, intramuscular injection and dipping to cultured olive flounder, Paralichthys olivaceus (average 300 g) at 20±1.0℃. The concentrations of AM in the plasma were determined by HPLC-UV detector. The average recoveries of AM in spiked samples between 0.01∼10 ppm were ranging from 84.45% to 91.26% for plasma. The Limit of detection for AM was 0.05 ppm by using this method. Plasma concentrations of AM were determined after oral dosage (10, 20 and 40 ㎎/㎏ body weight), intramuscular injection (5, 10 and 20 ㎎/㎏ body weight) and dipping (10, 20 and 40 ppm; 1 h). Samples were taken at 1, 3, 5, 10, 15, 24, 30, 48, 96, 144, 216, 264 and 360 h post-dose. In oral dosage of 10, 20 and 40 ㎎/㎏, it's peak plasma concentrations, which attained at 10 h post-dose in all doses, was 3.62±0.97, 5.20±0.70 and 11.18±0.87 ㎍/㎖, respectively. Those of AM were not measurable at 144, 360 and 360 h post-dose, respectively. In intramuscular injection of 5, 10 and 20 ㎎/㎏, it's peak plasma concentrations, which attained at 5 h post-dose in all doses, was 6.92±1.29 ㎍/㎖, 9.89±2.22 ㎍/㎖ and 19.85±2.97 ㎍/㎖, respectively. Those of AM were not measurable at 216, 264 and 264 h post-dose, respectively. In dipping of 10, 20 and 40 ppm, it's peak plasma concentrations, which attained at 3 h post-dose in all doses, were 4.39±1.10, 9.57±1.51 and 11.61±1.92 ㎍/㎖, respectively. Those of AM were not measurable at 264, 264 and 360 h post-dose, respectively. Therefore, the plasma distribution and elimination levels of AM in olive flounder were dosage-dependant manner in all administration routes.