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The study aimed to improve the injury belief, injury prevention behavior, and safety fulfillment confidence of teachers of infants and toddlers in childcare centers. The study subjects were 376 teachers who were assigned to either the intervention group or the control group. The intervention group participated in an Injury Prevention Program for infants and toddlers for 100 minutes once a week for a total of 5 sessions, while the control group did not. The collected data were statistically analyzed through the independent t-test and paired t-test. The injury belief and injury prevention behavior test scores of the teachers in the intervention group were significantly increased compared to those in the control group after the completion of the five-week program.