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Early life history of the endangered species Gobiobotia brevibarba was investigated to provide baseline data for biological characteristics and restoration. Adult fish were sampled by spoon net capture at Hongcheon-gun, Gangwon-do, Korea, in April 2010. Eggs were obtained after injection of Ovarprim and were artificially fertilized by the dry method in the laboratory. The mature eggs were slightly adhesive and transparent with greyish and average 1.24±0.07 mm in diameter. The hatching of the embryos took place about 120 h after fertilization at a water temperature of 23℃. The newly-hatched larva averaged 5.5±0.29 mm in total length. Four days after hatching,they averaged 7.4±0.26 mm in total length and their yolk sacs were completely absorbed. From 15 days after hatching, they entered the juvenile stage and reached 9.9±0.72 mm in total length. One hundred days after hatching, their band patterns and external form were similar to those of adults, and they averaged 36.0±3.11 mm in total length.