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The purpose of this study is to provide basic data for making mediation programs about enhancing women's physical activity. To this end we found out the effects of regular exercise and women's age on sub-factors of perceived exercise barrier factors. We surveyed 289 adult women on exercise barrier factors and categorized the results into age and regular exercise. Then, conducted two-way ANOVA to find out the effects of women's age and regular exercise on sub-factors of exercise barrier factors. As a result, we came up with the following. First, there was a significant difference among social and cultural barrier factors(free time, partner, costs, absence of leader) that are perceived whether the participants do exercise or not. Second, all elements in sub-factors of exercise barrier factors depending on the age group had statistical significance difference except disease(a sub-factor of physical barrier factors) and wheather(a environmental and geographical barrier factor. Third, the age and whether adult women exercise or not interact with all sub-factors except injury, hypertrophy, unnecessariness, fear, physical barrier factors, and air pollution, environmental and geographical barrier factor. From our study, barrier in exercise are different depending on the age and whether they exercise or not. Therefore when making mediation programs about enhancing physical activity, our results should be considered.