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The purpose of this study is to determine the expression of a acute endurance exercise both in cold(2°C) and hot(34°C) temperatures on the skeletal muscle. The main purpose of this study is to the effects of cold and hot temperatures to the heat shock protein 70, heat shock protein 27 and blood lactate, plasma CK using rats as the exercise. Adult male Sprague-Dawley rats were randomly placed in one of three temperature groups (normal, hot, cold). The treadmill run with an intensity of 26m/min, 10°intensity and soleus(SOL) and extensor digitorum longus(EDL) muscle extracted at 30, 60, 90 min during the exercise. The western blotting analyzed the expression of HSP70 and HSP27 during the exercise, the increased HSP27 protein in the SOL and EDL tissues of the cold, hot and normal temperatures were observed. However, it was observed that only SOL tissues increased HSP70 protein in both cold and hot temperatures. These results implied that increased apoptotic factor by cold and hot temperatures could cause the expression of HSP70 and HSP27 which involve cell damage in early stage of exercise and due to temperatures.