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The purpose of this study was to conduct long-term and short-term walking exercise programs on obese college women, and provide scientific data obtained from the programs for the prevention and treatment of obesity through walking. The mean and standard deviation for each variable are calculated using SPSS 18. for Windows, and Repeated measures ANOVA was used to see the change between the experimental group (24-week) and control group (12-week). The results are as follows: First, 24-week walking exercise program was effective in managing weight, body mass index(BMI), fat mass, muscle mass, and belly for prevention of adult disease in obese college women. Second, there was an increase in HDL-C and a decrease in TC through walking exercise program, more than 3 months, 3 days per week, and 1 hour per day. Third,long-term walking exercise program yielded a positive result in reducing systolic and diastolic blood pressure, and Leptin in obese college women. However, there was no positive result from the control group in reducing Leptin after 12-week walking exercise program; Therefore, there is a need for more intense,more frequent, and longer program.