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The purpose of this study was to investigate the mitochondria biogenesis and function of gracilis muscle following prolonged taekwondo training. In this study, taekwondo trained group (14±2.4yr, 4time week^-1,3-4hour/day^-1; EXE, n=6) and non regular exercise training group(NEXE, n=6) were participated and gracilis muscle(20mg) was extracted while ACL surgery with voluntary consent AMPK, pAMPK and CAMK protein which are intracellular messenger were significantly over expressed in EXE group compared with NEXE group. PGC-1α which is referred as main controlling protein for mitochondria biogenesis also remarkably expressed in EXE group. furthermore, UCP3 and GLUT4 protein expression in EXE group were significantly over expressed compared with NEXE group. As to these results, protein overexpression for mitochondria biogenesis and function of gracilis muscle following prolonged taekwondo training were adaptive phenomenon, which might be expressed as improved taekwondo performance because of not only higher anaerobic capacity but also higher aerobic capacity in elite taekwondo player