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The extrinsic glucose supply, the biosynthesis of UDP-GlcNAc, protein O-GlcNAcylation and sialylation of cell surface proteins are now increasingly important aspects for normal function of brain neuronal system. As a preliminary study, we investigated changes in protein O-GlcNAcylation, sialylation and the content of UDP-GlcNAc in the rat brain cortical tissues of different ages, embryonic(E18), new-born(P0), 4-week(P4W) and 8-week-old(P8W) rats. As the rats grow up to young adult(8-week-old), OGlcNAcylation in total proteins and number of protein species gradually increased along with sharp decrease in UDP-GlcNAc content. In contrast to O-GlcNAcylation, total sialic acid content in total proteins of each tissue showed an increase from E18 to P0 and then gradually decreased. These results may suggest that UDP-GlcNAc metabolism and protein O-GlcNAc modification and sialic acid expression are controlled by rather complicate mechanism during the normal development of early post-natal brain tissues. Elucidating the mechanisms of the metabolic processes between these factors and their regulation would be beneficial for the prevention and treatment of neurodegenerative diseases such as hereditary and Alzheimer’s diseases.