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In this research, for the first time, we tried to analyse Raman hyperspectral dentin data using Independent Component Analysis (ICA) to see its possibility of adoption for the dental analysis software. We captured hyperspectral dentin data on 569 spots on a molar with dental lesion by HR800 Micro Raman Spectrometer at UMKC-CRISP (University of Missouri at Kansas City-Center for Research on Interfacial Structure and Properties). Each spot has 1,005 hyperspectral data. We applied ICA to the captured hyperspectral data of dentin for evaluating ICA approach, and compared it with the well known multivariate analysis method, PCA. As a result of the experiment, ICA approach shows better local characteristic of dentin than the result of PCA. We confirmed that ICA also could be a good method along with PCA in the dental analysis software.