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Major Royal Jelly Protein 4 (MRJP4) is a member of Major Royal Jelly Protein (MRJP) family. In this study MRJP4 was cloned from cDNA of Apis mellifera, and recombinant MRJP4 was expressed and purified. Both DNA and amino acid sequences of MRJP4 in this studyrepresented 99% homology with reported sequence on GenBank (NM_001011610). The gene was sub-cloned into pET32a (+) to express recombinant protein. The protein was successfully expressed as inclusion body form and could be purified using His-tag column at denatured condition. Optimal condition for production of MRJP4 from E. coli was also determined by the tests of variable factors. Based on optimal condition, 3mg pure recombinant MRJP4 could beharvested from 1 liter culture of E. coli.