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This study examined the separation characteristics of particles in the rainfall runoff from paved roads using a φ7.5 cm hydrocyclone. The volume fraction and total suspended solids concentrations in the overflow and underflow from the hydrocyclone, as well as the separation efficiency were determined. The results indicated that the overflow volume increased with increasing operational pressure, but decreased with decreasing ratio of underflow outlet to inlet sizes (Du/Di), while the underflow to overflow volumes showed contrary behavior. The total suspended solid (TSS) concentration ratio between the overflow and inflow (TSSover/in) decreased as a function of the operational pressure, while the corresponding ratio of underflow to inflow (TSSunder/in) increased. There was no visible difference in the TSSover/in with increasing Du/Di ratio, but the TSSunder/in decreased sharply. The particle removal efficiency was mainly affected by the particle size.