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유도성 산화질소합성효소(inducible nitric oxide synthase, iNOS)는 다양한 생리작용에 관여함으로 많은 연구가 이루어져 있다. 그러나 형태학적 연구보고는 연구자에 따라 매우 달라서 iNOS의 발현에 대한 검토가 필요하다. 본 연구에서는 4개 회사(Chemicon, CH; Sigma, SI; Transduction Laboratories, TL; Upstate, UP)의 iNOS 항체의 면역염색결과를 비교하고, 모든 아형의 NOS에 대한 염색이 가능한 NADPH-diaphorase에 대한 광학적 및 전자현미경적 효소조직화학법을 병용함으로써 iNOS 발현을 확인하고자 하였다. SD계 흰쥐에 LPS를 투여하여 iNOS의 발현을 유도하였다. LPS 투여군에서 NADPH-d 전자현미경적 효소조직화학법의 결과 반응산물의 위치가 세포질기질에 있는 세포와 사립체에 있는 세포로 구별되었다. 반응산물이 세포질기질에 있는 세포는 치밀반점, 내림가는세관(DTL), 주머니상피(CE) 및 사이질의 유주세포(WC)이며, 대부분 세뇨관의 세포에는 사립체에 반응산물이 위치하였다. LPS 투여군에서 DTL, CE 및 WC가 염색된 항체는 TL과 UP이나, 사립체에 반응산물이 있는 세뇨관세포도 일부 염색되었다. 본 실험의 결과로서 NADPH-d 효소조직화학법을 병용하면 iNOS를 발현하는 세포를 정확하게 동정할 수 있을 것으로 생각된다


Inducible nitric oxide synthase (iNOS) has been known to be involved in the various physiological metabolim and has been attracting topic. However, there are extensive differences in the reports about the localization of iNOS expression. To resolve this discrepancy, we compared immunohistochemical data from four iNOS antibody produced by different company (Chemicon, CH; Sigma, SI; Transduction Laboratories, TL; Upstate, UP), and NADPHdiaphorase (NADPH-d) enzyme-histochemical results using light- and transmission electorn-microscope in the lipopolysaccharide (LPS)-treated rat kidney. Electron microscopical examination revealed two different distribution of the NADPH-d reaction product. In the majority of NADPH-d reaction-positive cells, reaction depositions were restricted to the mitochondia, and in the cells of macula densa, descending thin limb (DTL), capsular epithelium (CE) and interstitial wandering cells (WC), NADPH-d positivities were found in the cytoplasm. In immunohistochemical results from LPStreated animal, DTL, CE and WC were positively stained with TL and UP antibodies but with CH and SI antibodies. We conclude that NADPH-d histochemistry may be usefull for identifing the iNOS-positive cells morphologically.


Inducible nitric oxide synthase (iNOS) has been known to be involved in the various physiological metabolim and has been attracting topic. However, there are extensive differences in the reports about the localization of iNOS expression. To resolve this discrepancy, we compared immunohistochemical data from four iNOS antibody produced by different company (Chemicon, CH; Sigma, SI; Transduction Laboratories, TL; Upstate, UP), and NADPHdiaphorase (NADPH-d) enzyme-histochemical results using light- and transmission electorn-microscope in the lipopolysaccharide (LPS)-treated rat kidney. Electron microscopical examination revealed two different distribution of the NADPH-d reaction product. In the majority of NADPH-d reaction-positive cells, reaction depositions were restricted to the mitochondia, and in the cells of macula densa, descending thin limb (DTL), capsular epithelium (CE) and interstitial wandering cells (WC), NADPH-d positivities were found in the cytoplasm. In immunohistochemical results from LPStreated animal, DTL, CE and WC were positively stained with TL and UP antibodies but with CH and SI antibodies. We conclude that NADPH-d histochemistry may be usefull for identifing the iNOS-positive cells morphologically.