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The degradation of antibiotic tetracycline by using electron beam irradiation was investigated in an aqueous solution as a function of irradiation dose. The degradation efficiency of tetracycline was a level of 100% at an irradiation dose of 5 kGy. The degradation of tetracycline follows an apparent a “first order” reaction rate dependent on irradiation dose. Electron beam technology is suggested to be a way to degrade antibiotics and may be used as an approach for biological treatment of domestic and livestock wastewater.