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Objectives : In this study, the author tried to investigate whether Geonpye-tang(GPT) significantly affect PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production and gene expression from human airway epithelial cells. Materials and Methods : Effect of the agent on PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production and gene expression from human airway epithelial cells (NCI-H292) were investigated. Confluent NCI-H292 cells were pretreated for 30 min in the presence of GPT and treated with PMA (10ng/ml) or EGF (25ng/ml) or TNF-alpha (0.2nM), to assess both effect of the agent on PMA- or EGF- or TNF-alpha-induced MUC5AC mucin production by enzyme-linked immunosorbent assay (ELISA) and gene expression by reverse transcription-polymerase chain reaction (RT-PCR). Possible cytotoxicity of the agent was assessed by examining the rate of survival and proliferation of NCI-H292 cells after treatment of agent during 72 hrs (SRB assay). Results : (1) GPT significantly inhibit PMA-induced and EGF-induced MUC5AC mucin productions from NCI-H292 cells. However, GPT did not affect TNF-alpha-induced MUC5AC mucin production ; (2) GPT significantly inhibit the expression levels of PMA- or EGF- or TNF-alpha-induced MUC5AC gene in NCI-H292 cells ; (3) GPT did not show significant cytotoxicity to NCI-H292 cells. Conclusion : This result suggests that GPT can affect the production and gene expression of respiratory mucin observed in diverse respiratory diseases accompanied by mucus hypersecretion. This can explain the traditional use of GPT in oriental medicine. Effect of GPT with their components should be further investigated using animal experimental models that reflect pathophysiology of airway diseases through future studies.