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In this study, microcomputer-based experiments that helped to overcome the existing problems of photosynthesis and respiration experiments were developed. Developed experimentations were improved with regard to the selection of experimental materials(plants and animal), light source and sensors and were included decision of data collecting software, data collection time and data collecting rate. According to standards for selecting materials, light source and sensors, spinach leaves and crickets as experimental materials, a halogen lamp as a light source were selected. Data were collected every 4 seconds for 180 seconds using oxygen gas sensor and carbon dioxide gas sensor as a probe. Microcomputer based experiments on photosynthesis and respiration were expected to be a solution for the problems with the existing experiments on photosynthesis and respiration.