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This study was performed to assess gender differences in rates of obesity, dietary behaviors, and nutrient intakes among college students living in Gwangju. Anthropometric measurements showed that the body mass indexes (BMIs) of the males and females were 22.1±2.6 and 20.1±2.4 respectively. All obesity indices including BMI, relative body weight (RBW), % body fat by bioelectrical impedence analysis (BIA), and waist-hip ratio (WHR) were significantly higher in the males than in the females. Abdominal fat was also found to be higher in the males whereas underweight was prominent in the female students. The dietary behaviors of the males as determined by dietary scores, were poorer than those of the females. Twenty-four hour dietary recalls revealed that most nutrient intakes were adequate, exceptions of vitamin C, Ca, and folate intakes in both sexes and Fe intake in the female students. Ca and folate intakes were below 75% of the KDRI for both genders. In addition, Unbalanced energy ratios of carbohydrate, protein, and fat were noted in both genders. Effective nutrition education programs targeting college students should be developed and implemented to increase Ca and folate consumption. It is suggested that gender-based nutrition education approaches be created due to poor dietary behavior in males and inadequate nutrient intakes in female college students.