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Purpose: The purpose of this study was to identify the relationship of influenza A (H1N1) knowledge, attitude and practice for nurses. Methods: Data were collected by self-report questionnaires from a total of 325 nurses working in an Influenza A (H1N1) base-zone hospital in C city during September, 2009. The collected data were analyzed using of SPSS/WIN 17.0. Results: The knowledge of influenza A (H1N1) was not statistically different for gender, age, education, work unit, clinical experience, position, or previous education of Influenza A (H1N1). The attitude to influenza A (H1N1) was statistically significant according to age or clinical experience. Practice related to influenza A (H1N1) was statistically different for education, clinical experience or previous education of influenza A (H1N1). Knowledge of influenza A (H1N1) was lowest for etiology and definition compared to other subcategories. Attitude and practice were significantly different for all items. The biggest difference in items was for 'use of physical barriers (protective goggles, face masks and gowns) during procedures that may involve contact with aerosol'. There was a positive association between attitude and practice. Conclusion: An educational program focusing on strategy to change nurses's knowledge, attitude and practice can be effective for infection control in an influenza A (H1N1) base-zone hospital.