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In vitro mass multiplication of Vanilla planifolia was investigated using node as explant. Multiple shoots were developed in MS medium supplemented with 2.0 mg l-1 6-benzylaminopurine and 1.0 mg l-1 -naphthalene acetic acid. Multiple shoots were maintained for 6-7 weeks with regular subculturing at the end of 3rd week onto fresh medium. The maximum number of shoots at the rate of 12.8 per node segment was achieved over a period of four weeks. The elongated shoots were separated from the shoot clusters and were transferred onto half strength MS medium supplemented with indole-3-acetic acid (1.0 mg l-1) over a period of 28 days for induction of roots. The development of roots was observed on 7th day of incubation. The in vitro raised plantlets were transferred to poly-cups, covered with polyethylene sheets and maintained under shade net for 25 days for hardening. Finally these plants were transferred to field and recorded that 85 % of tissue cultured plants were survived. From the present study, a simple and efficient micropropagation protocol was developed for Vanilla planifolia using single node segments as explants.