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investigated effect of small black soybean fraction (SBSF) T cell-mediated responses for tumorsurveillance and proliferation in leukemia cells in vitrochloroform fraction (SBSFCh) was administered p.o. once a day for 21 days in BALB/c mice and then levels ofserum cytokines and subpopulation of lymphocytes were measured. Moreover, SBSF fraction was treated into thecultured various cell lines for proliferation in leukemia cell lines, NO production by RAW264.7 cells, andexpression of p53 gene in U937 leukemia cells. These results showed that SBSFBu increased levels of serum IL-4but not IL-2 and IFN-γ, and increased expresion of CD4+ T cells and CD8+ T cells in splenocytes in vivo, whileγ but decreased IL-4, and increased CD8+ T cells but not CD4+T cells. Moreover, both of SBSFBu and SBSFCh inhibited proliferation of HL60, U937, and L1210 leukemia celllines in a dose-dependent manner, up-regulated NO production by RAW264.7 cells in a dose-dependent manner,and enhanced expresion of p53 gene in U937 leukemia cells. Our findings indicate that SBSFBu and SBSFChmay enhance T cell-dependent immune responses, and that both of SBSFBu and SBSFCh may inhibitproliferation of leukemia cells by up-regulation of NO production and expression of p53 gene. KeywordsGlycine max, lymphokines, CD4+, CD8+, cell proliferation, NO, p53