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Background and Objectives:Neuronal dissociation culture is an important tol for the study of neuronal cel survival and ganglion neurons in vivo. This study was aimed to establish the culture systems for the spinal neuronal cells and to characterize the cultured cells using different neuronal marker. Materials and Methods:Dissociated spiral ganglion cells were harvested from Sprague Dawley rats in postnatal 5 or 6 days and cultured for 48 hours. To prepare in vivo section, the harvested cochlea was embedded in parafin and sectioned in 4 μm thickness. Both cultured cells and parafin sections were labeled with seve-ral monoclonal antibodies (NSE, NF-200, NF-160, S-10) unoflourescent staining methods. Results:Each of the antibodies was used to stain both cultured cells and parafin sections. NSE was used to stain the nuclei of neuronal cells. Either NS or NR was used to stain both neuronal perikarya and neurite. The Schwan cells were stained by S-100. There was no significant difference in the immunostaining pattern between cochlea tissue and dissociated cells of spiral ganglion. Conclusion:A dissociated culture system for the spiral ganglions was established. In system can be used for the study of neuronal cell biology. (Korean J Otolaryngol 2002;45:433-8)