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Background and Objectives:Fibroblasts interact with eosinophils and play a key role in the pathogenesis of airway dise-ases. The aims of this study were to investigate whether Th1 or Th2 type cytokines can induce mRNA and protein expresion of eotaxin and RANTES in human nasal fibroblast (HNF) and to verify the corelation betwen the stimulation of different cytokines and chemokines in HNF. Materials and Methods:Cultured HNF were stimulated by IL-13, TNF-α, IFN-γ, IL-13 with TNF-α, IL-13 with IFN-γ, TNF-α with IFN-γ >?F??(??(?F)?? (0.2, 2, 20, 200 ng/ml). MRNA expression of eotaxin and RANTES were revealed by RT-PCR and protein of eotaxin and RANTES were revealed by ELISA. Results:TNF-α and IFN-γ induced mRNA and protein expr-esion of RANTES in HNF and they synergisticaly induced protein expresion of RANTES. RANTES expresion increased in a time dependent manner. IL-13 induced mRNA and protein expresion of eotaxin in HNF and it synergisticaly reacted with TNF-α or IFN-γmanner. Conclusion:Th1 or Th2 type cytokines induce mRNA and protein expresion of eotaxin and RANTES in human nasal fibroblasts. TNF-α or IFN-γ induce more RANTES than eotaxin but IL-13 induces more eotaxin than RANTES. There may be some synergic effects of cytokines for mRNA and protein expresion of chemokines. (Korean J Otolaryngol 2002;45:467-72)