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This study was to examine changes of electroencephalogram (EEG) of frontal and state anxiety when EEG bio-feedback training was conducted with five rifle shooters of girls' high school. Experiment was performed for 30 min per session, four times a week through eight weeks and two measurements before and after training at stable state were conducted. Measurement of EEG used FP1, FPz and FP2 attached to head band of Neuro-Feedback System (Braintech Corp., Korea) and left and right brain powers of theta, alpha, SMR (sensory motor rhythm) and beta and ratio of alpha/SMR wave were analyzed. As a result of analysis, there were significant decreases at theta, alpha, SMR and beta waves of left brain before and after experiment and right brain also showed decrease of theta, alpha, SMR and beta waves, but there was no statistically significant difference. Relative activation state of alpha/SMR wave rates showed significant increase of alpha wave at left brain before and after experiment and then it is found that alpha wave reflecting stable state compared to SMR wave related to attention was influential. And state anxiety showed a significant decrease before and after experiment and competition performance showed no significant difference. These results suggest that application of EEG bio-feedback training was very helpful to make brain functions in shooters who required high technique, precision, mental stability and self-control exert more affirmative abilities and improve their exercise learning. Further, it is considered that additional researches should be performed on the basis of the analysis of changes of real-time EEG and physio-psychology in athletes during shooting.