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Bone resorption surrounding tooth root causes tooth loss in periodontitis patients. Osteoclast has bone resorptionactivity. Effects of Artemisia asiatica on bone resorption induced by periodontopathogens, Porphyromonas gingivalis andTreponema denticola, were examined using co-culture systems of mouse osteoblasts and bone marrow cells. Addition of A.asiatica ethanol extract to bacterial sonicate abolished bacteria-induced osteoclastogenesis. To determine inhibitory mechanism ofA. asiatica against osteoclastogenesis, effects of A. asiatica on expressions of osteoclastogenesis-inducing factors such asreceptor activator of NF-κB ligand (RANKL), prostaglandin E2 (PGE2), interleukin (IL)-1, and tumor necrosis factor (TNF)-α,in osteoblasts were examined. A. asiatica suppressed expressions of RANKL, PGE2, IL-1β, and TNF-α increased by eachbacterial sonicate. These results suggest inhibitory action of A. asiatica against osteoclastogenesis is associated with downregulationsof RANKL, PGE2, IL-1β, and TNF-α expressions.