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Rats were fed with cholesterol-free diet containing 5 g/100 g cellulose powder (CP), adzuki fiber (AF), kintoki fiber(KF), or tebou fiber (TF) for 4 weeks. Serum cholesterol levels of bean fiber groups were not significantly different from that ofCP group at the end of feeding period. Hepatic cholesterol concentration of CP group did not significantly difter from those offiber groups. Fecal cholesterol concentration of CP group was higher than that of AF group. Fecal bile acid concentration of CPgroup was significantly higher than those of AF and TF groups. No significant differences were observed in relative quantity ofhepatic cholesterol 7α-hydroxylase mRNA levels among groups. These results revealed that dietary fibers of adzuki, kintoki, andtebou beans did not act as dietary factor lowering serum cholesterol concentration in rats fed with cholesterol-free diet.