초록 close

Optimal extraction conditions such as pressures, temperatures, and modifiers on glycyrrhizin extraction fromlicorice were investigated using supercritical CO2 (SC-CO2) at 3 mL/min flow rate. Morphology of licorice tissue, afterglycyrrhizin extraction, was examined by SEM, and absolute density (g/cm3) measurement and glycyrrhizin content weredetermined by HPLC. Pure SC-CO2 had no effect on glycyrrhizin extraction, but recovery of glycyrrhizin (32.66±0.77%) wasenhanced when water was used as modifier. The highest recovery was 97.22±2.17% when 70% (v/v) aqueous methanol wasadded to 15% (v/v) SC-CO2 at 50 MPa and 60oC. Under optimal extraction conditions, 30 MPa pressure and 60oC heatingtemperature, glycyrrhizin recovery reached maximum (102.67±1.13%) within 60 min. Licorice tissue was severely damagedby excessive swelling, and absolute density of licorice residues was highest when aqueous methanol was used as a modifier.