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For sensory evaluation of food or personal products which have a considerable carry-over or fatigue, it is necessary to apply appropriate resting or mouthrinsing between the testing. However, interstimulus delay caused by such resting or mouthrinsing increases memory load as well as the session length required for the test. Therefore, as a solution to this problem, an alternative technique, bi-lateral tasting has been examined in comparison to normal full mouth testing. Three 2-AFC procedures with different memory requirements were used to investigate the most efficient method for assessing the irritating sensation of toothpaste: a full mouth brushing with 20 min rest (interstimulus interval) between toothpastes, a full mouth brushing with no rest between toothpastes, and a bi-lateral brushing. Significant carry-over effects were observed in toothpaste evaluation. The adapted bi-lateral testing resulted the highest sensitivity and appeared to be applicable for toothpaste evaluation with the advantage of taking less time with higher sensitivity.