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The purpose of this study was to find out educational effects of project learning about ‘The diversity of life and Environment' in biological class. For this study, I designed students-centered project learning materials. These materials were made of 3 steps; introduction, small group activity, presentation of their own products. Students had to do a mission for 6 weeks. That was so called ‘create creature'. 2 students made a group and each group selected its own ecosystem. The groups created their own creature in various ecosystem. At first week, teacher introduced the relation of life and environment. At second week, each group aggregated some information about its own ecosystem and animals from textbook, science magazine, internet etc. At third week, each group had made its own creature that well adapted themselves to its own ecosystem. At the last week, each group presented their products. In this study, 54 students in the 11th grade of the science high school were implemented. The results of this study was as follows first, most of students showed great interests and enjoyed the class of this style, and they thought it was a good chance for creative thinking; second, it was easy to understand about the animals in each ecosystem; third, the results of test of science related attitudes(TOSRA) in 6 of 7 domains were significantly different at pre-post test. From these, we concluded that project learning facilitated meaningful learning and scientific attitude.