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The Purpose of this study was to examine the effect of dietary fiber supplementation and regular exercise on body composition concerning obesity, Blood lipid and Defecation satisfaction. 30 female university students participated as subject and were randomly assigned to three group(exercise group; GroupⅠ, dietary fiber supplementation group; GroupⅡ and exercise with dietary fiber supplementation group; GroupⅢ). GroupⅠ and GroupⅢ intook 5capsules of dietary fiber product a day and GroupⅡand GroupⅢ performed complex exercise program consisted of 10min's warm up, 30min's treadmill running and 20min's weight training every another day during 6weeks. The pre and post value of body weight, %bodyfat, WHR, TC, TG and Dafecation satisfaction were measured to analyze the effect of each treatment. First, body weight, %bodyfat and WHR were decreased significantly in groupⅠ, groupⅡ and GroupⅢ after 6weeks' treatment, but there weren't any significant differences among group. Second, TC and TG level were decreased significantly in groupⅠ, groupⅡ and GroupⅢ after 6weeks' treatment, but there weren't any significant differences among group. Third, Defecation satisfaction measured using questionnaire incresed significantly in groupⅠ, groupⅡ and GroupⅢ after 6weeks' treatment, but there weren't any significant differences among groupThe Study suggest that dietary fiber supplementation with regular exercise should be recommended as effective prescription for person with obesity and defecation difficulty.