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- The present study was conducted to investigate the translocation of polyphenols, especially catechinderivatives, from mushroom medium mixed with green tea residues into fruiting body of Pleurotus eryngii. Pleurotuseryngiiwas grown on the media incorporated by mixing or surface-treated with dry materials including leaf petioles andyoung stems or leaves of green tea. The dry materials treated in medium did not affect plant height and fresh weight ofPleurotus eryngiibody. From the samples of Pleurotus eryngii, the eight main catechin derivatives (-)-gallocatechin(GC), (+)-catechin (C), (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epigallocatechin gallate (EGCG), (-)-gallocatechin gallate (GCG), (-)-epicatechin gallate (ECG), and (-)-catechin gallate (EGCG), and caffeine were analyzedquantitatively by HPLC. The results showed that EGC in Pleurotus eryngiiwas 45% more detected, when incorporatedwith the dry materials, than untreated control. Especially, content of EGCG was increased in surface-treated Pleurotuseryngiiup to 3.2 ppm, while it was not detected or reduced in control and other treatments. Caffeine content was greatlyincreased regardless of treatment method, compared with control (0.1ppm), showing 44 fold-amount in Pleurotus eryngiiat early growth stage when incorporated with the dry materials into medium. The results indicates that functionalcatechin derivatives of green tea would be partly translocated into Pleurotus eryngiithrought incorporation and surfacetreatment with residues of green tea plants.Key words - Pleurotus eryngii, Green tea, HPLC, Catechin derivatives, Caffein