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Korean mistletoe (Viscum album L) extract has been found to posses immunoregulating activity. In this study, Korean mistletoe extract, M11C (non-lectin components), was used to know whether this extract activates splenocytes to secret interleukin 1β (IL-1β). The splenocytes were treated with M11C, and then collected the supernatant and cell lysate that were prepared to analyze the level of IL-1β, using ELISA, immunoblotting, and RT-PCR. Maximum effective dose and time of M11C on IL-1β secretion from splenocytes were 200㎍/㎖ and 8 hours, respectively. Treatment dose and time for the maximum expression of IL-1β mRNA were 200㎍/㎖ and 4 hours, respectively. Saccharide degradation enzyme Viscozyme L completely blocked the effect of M11C on IL-1β secretion from splenocytes. As the result, among non-lectin components saccharide could be regarded as a main component for IL-1β expression from splenocytes.