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It is well-known that Korean mistletoe (Viscum album) extract has an immune activity and anticancer effect. In this study, Korean mistletoe extract, M11C (non-lectin components), was used to examine whether this extract might activate human peripheral monocyte to produce tumor necrosis factor-α (TNF-α). To examine the effect of M11C on the production of TNF-α from monocyte, the monocyte were stimulated by the M11C, and then collected the supernatant (M11C stimulated monocyte-conditioned media; MCM). MCM was treated to the TNF-α sensitive L929 cells, and then L929 cytotoxicity was measured by means of MTT. MCM had cytotoxic effect on L929. And the cytotoxic effect of MCM on L929 was almost abolished by anti-TNF-α antibody. These data indicated that MCM contained TNF-α, suggesting the TNF-α generation from M11C-stimulated monocyte. This suggestion was confirmed from the data that TNF-α was highly detected in MCM by immunoblotting technique. M11C effect on TNF-α production from monocyte was in the dose and stimulating time dependent manners. Also the effect of M11C on the expression of TNF-α mRNA from monocyte was shown in the dose and stimulating time dependent manners. As a result, Korean mistletoe extract, M11C, could be used for an immunostimulator.