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For the development of qualitative and quantitativePCR methods of genetically modified (GM) pepper developedin Korea, a capsanthin-capsorubin synthase (CCS) gene wasused as the endogenous reference gene. The primer pair ccs-F/R amplifying the pepper endogenous gene gave rise to anamplicon of 102 bp. No amplified product was observedwhen DNA samples from 16 diferent plants were used astemplates. The construct-specific primer pairs amplifying thebar gene and Ti7 introduced in GMpeper gave rise to an amplicon of 182 bp. Quantitative PCRasay was performed using a TaqMan probe and a standardplasmid as a reference molecule, which contained both anendogenous and event-specific sequence. For the validationof this method, the test samples containing 0.1, 1, 3, 5, and10% GM pepper were quantified.