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This study investigated the effects of variousculture parameters (carbon sources, temperature, initial pH ofculture, NaCl concentration, and light) on the growth andcanthaxanthin production by Dietzia natronolimnaea HS-1.growth and canthaxantin production was glucose, and thebest pH and temperature were 7 and 31oC, respectively. Inadition, the biomas and canthaxanthin production increasedin a medium without NaCl and in the presence of light.Under the optimized conditions, the maximum biomass, totalcarotenoid, and canthaxanthin production were 6.12±0.21 g/l,4.51±0.20 mg/l, and 4.28±0.15 mg/l, respectively, in anand 5.29 mg/l, respectively, in a batch fermenter system.