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The purpose of this study was to investigate the evaluation of regional antagonism in children and adolescents in Youngnam and Honam region. There were 684 subjects included in this study from three ages in the Youngnam and Honam regions: 12.8 years (6th grade, n=208), 14.8 years (8th grade, n=211), and 16.9 years (10th grade, n=265). Of the 684 subjects, 357 (53.3%) were boys and 327(47.8%) were girls. The instruments consisted of subjects' self-reported social distance, stereotype, and prejudice. The data were analyzed by frequencies, t-test, one-way ANOVA and the Scheff test. Major findings were as follows: The mean score of the out-group stereotype was greater than the median, so the in-group preference was greater than the out-group exclusion. The in-group stereotype was different based on the age and the sex. The 6th and 8th graders and the boys were higher than 10th and the girls. The prejudice was different based on the region. The social distance was different based on the age and the region.