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We investigated the effects of washing on the mechanical properties and hand values of spandex/cotton and cotton single jersey fabrics. These knitted fabrics were subjected to 30 laundering cycles with a drum-type washer and were followed by drying at 65oC with a tumble dryer. The mechanical properties of the cotton knitted fabrics changed greatly during the first washing and then suffered no further change in their properties as the number of washing cycle increased. However, the properties of the spandex/cotton knitted fabrics changed continuously throughout all the washing cycles. The cotton/spandex knitted fabrics showed higher values in tensile and bending properties than did the cotton knitted fabrics, though both kinds of fabrics showed less difference in shearing, surface and compression properties. The spandex/cotton knitted fabrics which had proper density had a higher THV for winter underwear after 30 repeated washings than did the cotton knitted fabrics, and the THVs of both fabrics decreased with the increase in the number of washing cycles.