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The objective of this study is to examine the effect of the coagulant and homogenization in bulking sludge thickening of paper manufacturing plant using DAF (Dissolved Air Flotation) and gravitational sedimentation. The effects of parameters such as dosage of coagulant and homogenization time were examined. The results showed that DAF and sedimentation was affected aluminum sulfate and anion polymer coagulant differently. At the optimum dosage of aluminum sulfate, thickening efficiency of DAF and sedimentation process were increase 1.25 time and 2.02 time, respectively. At the optimum dosage of anion polymer coagulant, thickening efficiency of DAF process was increase 1.35 time, but thickening efficiency at sedimentation was 1.06 time. When anion polymer coagulant of 0.5mg/l was added in DAF process, water content of sludge was decreased from 96.6% to 90.7% in dewatering process using Buchner funnel test device. After homogenization (20500 rpm, 10 min), sauter mean diameter of sludge floc was decreased from 631m to 427m, however increase of flotation efficiency by DAF was only 1.09 time.