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Nowaday many researches has proved that glutaradehyde(GA) is more excellent medicament in vital pulpotomy practice than formocresol(FC). But a number of dental practitioner prefer to use formocresol in vital pulpotomy procedure todays. And thus author reevaluate properties of gluteraldehyde and formocresol through implantation into epidermis and trypsin digestion after fixation at 2% buffered glutaraldehyde and Burkley's formocresol solution.. And then prepared tissues were stained by H&E and Masson-Trichome method. GA showed definite fixative zone and very low diffusible property in epidermis and pulp tissue as glutaraldehyde and formocresol were compared. GA represented exceedingly lower antigenicity than FC. GA fixation exhibited more resistibility to trypsin digestion than FC. As considering these results, it concluded that GA would be extremely superior medicament to formocresol in vital pulpotomy procedure.