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Culture conditions for generation of functional engineered tissues are designed to perform at least one of the following functions: (1)provide ecient mass transfer to the growing tissues. In this study, we have investigated the eectiveness of a perfusion culture system forconstruction of tissue-engineered soft tissues (TESTs). TESTs were constructed on poly(D,L-lactide-co-glycolic acid) (PLGA) scaoldswith HDFs. We compared biological and mechanical properties of TESTs constructed by static and perfusion culture conditions.The TESTs generated by perfusion culture revealed signicantly higher DNA (p < 0.01), GAG content (p < 0.01), and elastic modulus(p < 0.05) than TESTs constructed by static culture. Moreover, homogeneous distribution of HDFs and ECM throughout the scaoldwas noted in TESTS constructed by perfusion culture, rather than spatial variation, which was observed in TESTs constructed by staticculture. In an in vivo study, increased tissue regeneration was observed in TESTs fabricated by perfusion culture. Therefore, it is sug-gested that the perfusion culture system can constitute a more promising approach for constructing functional TESTs.