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The effects of initial concentration and pulsedpressurization on the inactivation of Clostridium sporogenesspores suspended in deionized water were determined duringthermal processing (TP; 105oC, 0.1 MPa) and presure-assisted thermal processing (PATP; 105oC and 700 MPa)Diferent inoculum levels (104, 106, and 108 CFU/ml) of C.sporogenes spores suspended in deionized water were treatedat 105oC under 700 MPa with single, double, and triple pulses.Thermaly treated samples served as control. No statisticalsignificances (p>0.05) were observed among al differentinoculum levels during the thermal treatment, whereas theinactivation rates (k1 and k2) were decreased with increasingthe initial concentrations of C. sporogenes spores during thePATP treatments. Double- and triple-pulsed pressurizationreduced more effectively the number of C. sporogenes sporesthan single-pulse presurization. The study shows that thespore clumps formed during the PATP may lead to an increasein pressure-thermal resistance, and multiple-pulsed pressurizationcan be more effective in inactivating bacterial spores. Themechanisms with regard to inoculum level and pulsedpressurization.