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The purpose of this study is to investigate the relationship between nurslng students' self-differentiation, their stress level and stress coping strategies. And following research questions were established. First, what is the difference of the stress level depending on the self-differentiation? Second, what is the difference of stress coping strategies in accordance with the self-differentiation? Participants for this study were 159 students selected from the 1st grades of G University, located in Incheon Metropolitan city. SPSS 10.0 statistic program was taken for drawing its frequency, percentage, average and standard deviation. And t-test, F-test, correlation and multiple regression were conducted. The results are summarized as follows: 1. The mean score for the level of self- differentiation, level of stress, level of stress coping strategies were 3.59, 2.50, 3.35. 2. The relationship between self-differentiation and stress level revealed a negative significant correlation 3. The relationship between self-differentiation and stress coping strategies revealed a no significant correlation. 4. The relationship between stress level and stress coping strategies revealed a no significant correlation. Conclusion: The results of investigating the variation differences depending on stress level, self-differentiation, and stress coping strategies were revealed self-differentiation and stress coping strategies explained stress level by 28.6%.