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Purpose: The purpose of this study was to investigate the influencing factors of interpersonal relationship in nursing college students for effective learning ability and teaching strategy. Method: In order to get the data by self-questionnaire, 166 subjects were selected. The instruments for this study were Preceptual Orientation Scale, Self-Efficacy Scale, Narcissistic Personality Disorder Scale, and Interpersonal Relationship Scale. The dada was analyzed by percentage, mean, standard deviation, t-test, one-way ANOVA, Scheffe' test, Pearson's correlation coefficient and Stepwise multiple regression using SPSS 12.0 program. Result: The main factors that affect interpersonal relationship were self-perception and social-efficacy. These variables were account for 37.9% of interpersonal relationship. The significant influencing factors on interpersonal relationship were self-perception, social-efficacy. Conclusion: It is necessary to develop a strategy to get positive perceptual orientation and successful interpersonal relationship for nursing college students by further studies with small group program for the best result.