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mecA,femA, and nuc genes was developed for the detection ofmethicillin resistance genes harbored by some Staphylococcusaureus isolates and for the simultaneous identification of suchisolates at the species level. The triplex PCR revealed thepresence of the femA and nuc genes in all the S. aureusisolates examined (n=105). Forty-four clinical isolates weremecA mecA positive.The PCR results had a 98 or 99% correlation with the resultsof PBP2a latex agglutination tests or oxacillin susceptibilitytests, respectively.