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Purpose: This study was to explore the clinical competence according to clinical ladder of operating room nurses. Method: The subjects were 125 the operating room nurses working at five university hospitals in four city. The instrument to measure the clinical competency and clinical performance of OR nurses was developed by researcher. It consisted of 12 domains of clinical competence and 23 items of clinical performance. Results: Clinical competence and clinical performance of OR nurses group devided into 4 groups by clinical ladder such as 0-12month, 13-36 mon. 37-84 mon. over 85 month were significantly different. More experienced nurses performed higher level of clinical performance and competency in 23 items and 12 domains. Conclusion: It will be needed to add concrete behavioral patterns and behavioral indicators of nursing competencies, per stage of the clinical ladder, by repetitive studies on nurses of various hospitals and to confirm the validity.